The cDNA sequence of human NAMPT was amplified by PCR from the pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, College of Missouri). The PCR merchandise have been digested and cloned into a pET21a+ vector applying NdeI and XhoI restriction enzymes. A point mutation was released by the quick change https://sergiosxchk.blogsmine.com/27001007/not-known-factual-statements-about-xeruborbactam