The piston is not Within the pipette shaft but Within the pipette suggestion. There is absolutely no air cushion in between piston and liquid. The piston contacts the liquid specifically, and no air is replaced for the duration of aspiration. Clockwise from top still left: various measurements of micropipettes; a https://types-of-pipette-accordin53074.designi1.com/51301396/the-greatest-guide-to-types-of-pipette-bulbs