Pour the mixture of lysate and Ni‐NTA agarose to the column, and allow it to empty both by gravity circulation or by applying a vacuum to The underside of your column. This function is licensed beneath a Resourceful Commons Attribution 3.0 Unported License. The images in this post are included https://www.facebook.com/NewTechFind/posts/pfbid02CsD3AaVcSkRwFf5Xd4E45XYBXLEW11uKWALk2WS43BHgfobUpcD4tRuHLPRx9NCvl