Pour the combination of lysate and Ni‐NTA agarose in the column, and allow it to empty possibly by gravity stream or by making use of a vacuum to the bottom from the column. Skip to main content material Thanks for checking out nature.com. You happen to be using a browser https://www.quora.com/How-toxic-are-Grignard-Reagents/answer/Even-Sa?prompt_topic_bio=1